The Impact of Biotic and Abiotic Stress Factors on Development of European Ash Tissue Cultures

Fraxinus excelsior L. is threatened by a variety of environmental factors causing decline the species. The most important biotic negatively affecting condition F. population are fungi such as pathogen Hymenoscyphus fraxineus. Abiotic with potentially harmful effect to accumulation heavy metals and salinity in soils. Thus, aim this study was investigate impact selected abiotic stress determine which them pose threat European ash. conducted using vitro techniques based on callus seedlings regenerated via indirect organogenesis. Tissue cultures exclude influence other factors, including ash extinction. results confirmed very strong pathogenic potential H. fraxineus after 14 days tissue cells died failed activate its defense mechanisms. Experiments showed high toxicity cadmium concentration 0.027 mmol/L. Salinity caused activity oxidation enzymes vary among calluses control suggesting play role controlling morphogenetic development cultures.